Skip to main content
Addgene

pSB4C5_J23101-CocE
(Plasmid #128129)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128129 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSB4C5 (iGEM registry)
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 7 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CocE
  • Alt name
    Cocaine esterase (EC: 3.1.1.84), UniProtKB - Q9L9D7
  • Species
    From Rhodococcus sp, codon optimized for E. coli
  • Promoter J23101

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI, golden gate (unknown if destroyed)
  • 3′ cloning site BsaI, golden gate (unknown if destroyed)
  • 5′ sequencing primer ATACGCAAGGCGACAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB4C5_J23101-CocE was a gift from Jean-Loup Faulon (Addgene plasmid # 128129 ; http://n2t.net/addgene:128129 ; RRID:Addgene_128129)

  • For your References section:

    Metabolic perceptrons for neural computing in biological systems. Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, Faulon JL. Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0. 10.1038/s41467-019-11889-0 PubMed 31462649
Related items:
Commonly requested with: