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pSB4C5_pBen-BenR
(Plasmid #128127)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128127 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB4C5 (iGEM registry)
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 7 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BenR
  • Species
    Pseudomonas putida
  • Insert Size (bp)
    957
  • Promoter pBen

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI, golden gate (unknown if destroyed)
  • 3′ cloning site BsaI, golden gate (unknown if destroyed)
  • 5′ sequencing primer ATACGCAAGGCGACAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a single C nucleotide insertion in the pBen promoter sequence. It is not known if this mutation affects plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB4C5_pBen-BenR was a gift from Jean-Loup Faulon (Addgene plasmid # 128127 ; http://n2t.net/addgene:128127 ; RRID:Addgene_128127)

  • For your References section:

    Metabolic perceptrons for neural computing in biological systems. Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, Faulon JL. Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0. 10.1038/s41467-019-11889-0 PubMed 31462649
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