sgRNA AAVS1
(Plasmid
#128119)
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PurposeCo-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128119 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAAV
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Vector typeMammalian Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA AAVS1 + GFP
- Promoter RSV/U6
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GFP_fw (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sgRNA AAVS1 was a gift from Dominik Niopek (Addgene plasmid # 128119 ; http://n2t.net/addgene:128119 ; RRID:Addgene_128119) -
For your References section:
Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity. Aschenbrenner S, Kallenberger SM, Hoffmann MD, Huck A, Eils R, Niopek D. Sci Adv. 2020 Feb 5;6(6):eaay0187. doi: 10.1126/sciadv.aay0187. eCollection 2020 Feb. 10.1126/sciadv.aay0187 PubMed 32076642
