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Addgene

Cas-Acr (wtAcrIIA4)
(Plasmid #128115)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128115 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CascAID (wtAcrIIA4)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cas-Acr (wtAcrIIA4) was a gift from Dominik Niopek (Addgene plasmid # 128115 ; http://n2t.net/addgene:128115 ; RRID:Addgene_128115)

  • For your References section:

    Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity. Aschenbrenner S, Kallenberger SM, Hoffmann MD, Huck A, Eils R, Niopek D. Sci Adv. 2020 Feb 5;6(6):eaay0187. doi: 10.1126/sciadv.aay0187. eCollection 2020 Feb. 10.1126/sciadv.aay0187 PubMed 32076642
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