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AcrIIC1X* (AcrIIC1X-mCherry chimera)
(Plasmid #128114)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128114 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AcrIIC1X* (AcrIIC1X-mCherry chimera) was a gift from Dominik Niopek (Addgene plasmid # 128114 ; http://n2t.net/addgene:128114 ; RRID:Addgene_128114)

  • For your References section:

    Computational design of anti-CRISPR proteins with improved inhibition potency. Mathony J, Harteveld Z, Schmelas C, Upmeier Zu Belzen J, Aschenbrenner S, Sun W, Hoffmann MD, Stengl C, Scheck A, Georgeon S, Rosset S, Wang Y, Grimm D, Eils R, Correia BE, Niopek D. Nat Chem Biol. 2020 Apr 13. pii: 10.1038/s41589-020-0518-9. doi: 10.1038/s41589-020-0518-9. 10.1038/s41589-020-0518-9 PubMed 32284602
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