AcrIIC1-mCherry chimera #10
(Plasmid
#128113)
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PurposeExpresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-N1
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Vector typeMammalian Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcrIIC1-mCherry chimera #10
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-fw (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AcrIIC1-mCherry chimera #10 was a gift from Dominik Niopek (Addgene plasmid # 128113 ; http://n2t.net/addgene:128113 ; RRID:Addgene_128113) -
For your References section:
Computational design of anti-CRISPR proteins with improved inhibition potency. Mathony J, Harteveld Z, Schmelas C, Upmeier Zu Belzen J, Aschenbrenner S, Sun W, Hoffmann MD, Stengl C, Scheck A, Georgeon S, Rosset S, Wang Y, Grimm D, Eils R, Correia BE, Niopek D. Nat Chem Biol. 2020 Apr 13. pii: 10.1038/s41589-020-0518-9. doi: 10.1038/s41589-020-0518-9. 10.1038/s41589-020-0518-9 PubMed 32284602