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Addgene

pCAG-HA-Nrxn1α-LNS5
(Plasmid #128109)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128109 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone manufacturer
    Addgene Plasmid #58266
  • Backbone size w/o insert (bp) 4869
  • Total vector size (bp) 5679
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nrxn1a-LNS5
  • Alt name
    Nrxn1a LNS5 domain
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    819
  • Entrez Gene
    Nrxn1 (a.k.a. Nrxn1b)
  • Promoter CAG
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HA-Nrxn1α-LNS5 was a gift from Huda Zoghbi (Addgene plasmid # 128109 ; http://n2t.net/addgene:128109 ; RRID:Addgene_128109)

  • For your References section:

    Neurexophilin4 is a selectively expressed alpha-neurexin ligand that modulates specific cerebellar synapses and motor functions. Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown AM, Lin T, Chen W, Xue M, Sillitoe RV, Jiang X, Zoghbi HY. Elife. 2019 Sep 16;8. pii: 46773. doi: 10.7554/eLife.46773. 10.7554/eLife.46773 PubMed 31524598
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