pCMV-Nxph4-4Q-HA
(Plasmid
#128104)
-
PurposeExpress HA tagged Nxph4-4Q
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 128104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV
-
Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 3718
- Total vector size (bp) 4675
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNxph4
-
Alt nameNxph4 with four asparagine glycosylation sites mutated to glutamine
-
SpeciesM. musculus (mouse)
-
GenBank IDNM_183297.2
-
Entrez GeneNxph4 (a.k.a. 1110036M10Rik, AI851051)
-
Tag
/ Fusion Protein
- HA
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV-Nxph4-4Q-HA was a gift from Huda Zoghbi (Addgene plasmid # 128104 ; http://n2t.net/addgene:128104 ; RRID:Addgene_128104) -
For your References section:
Neurexophilin4 is a selectively expressed alpha-neurexin ligand that modulates specific cerebellar synapses and motor functions. Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown AM, Lin T, Chen W, Xue M, Sillitoe RV, Jiang X, Zoghbi HY. Elife. 2019 Sep 16;8. pii: 46773. doi: 10.7554/eLife.46773. 10.7554/eLife.46773 PubMed 31524598