DinoIII
(Plasmid #128100)

• Purpose: Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.
• Depositing Lab: Senjie Lin
• Publication: Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: microorganisms8010126. doi: 10.3390/microorganisms8010126.

Backbone

• Vector backbone: pMD19 T-Vector
• Backbone manufacturer: Takara
• Backbone size w/o insert (bp): 2679
• Total vector size (bp): 5137
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RNA complex & upstream/downstream of LHC
• Species: Dinoflagellates Karenia brevis and Fugacium kawagutii
• Insert Size (bp): 2458
• GenBank ID: FJ434727
• Promoter: Inserts are potential promoters

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry