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Addgene

HM3C NaVSp1 pET24b
(Plasmid #128088)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128088 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NaVSp1p
  • Species
    Silicibacter pomeroyi
  • GenBank ID
    AY392137.1
  • Tags / Fusion Proteins
    • His Tag
    • MBP
    • 3C Cleavage

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HM3C NaVSp1 pET24b was a gift from Dan Minor (Addgene plasmid # 128088 ; http://n2t.net/addgene:128088 ; RRID:Addgene_128088)

  • For your References section:

    Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins. Shaya D, Kreir M, Robbins RA, Wong S, Hammon J, Bruggemann A, Minor DL Jr. Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12313-8. doi: 10.1073/pnas.1106811108. Epub 2011 Jul 11. 10.1073/pnas.1106811108 PubMed 21746903
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