HA tag NaVSp1 FL pIRES
(Plasmid
#128087)
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PurposeMammalian cell expression vector for NaVSp1 bacterial sodium channel. N-terminal HA tag.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128087 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRES
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNaVSp1
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SpeciesSilicibacter pomeroyi
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GenBank IDAY392137.1
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV For (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA tag NaVSp1 FL pIRES was a gift from Dan Minor (Addgene plasmid # 128087 ; http://n2t.net/addgene:128087 ; RRID:Addgene_128087) -
For your References section:
Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation. Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL Jr. Cell. 2016 Feb 25;164(5):922-36. doi: 10.1016/j.cell.2016.02.001. 10.1016/j.cell.2016.02.001 PubMed 26919429
