NaVAe1 Sp1 CTD pIRES
(Plasmid
#128085)
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PurposeMammalian cell expression vector for (NaVAe1 1-243 NaVSp1 220-255) chimera bacterial sodium channel.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128085 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIRES
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Backbone manufacturerClonetech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNaVAe1 and Sp1
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SpeciesSilicibacter pomeroyi/Alkalilimnicola ehrlichii
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GenBank IDAY392137.1 / CP000453.1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NaVAe1 Sp1 CTD pIRES was a gift from Dan Minor (Addgene plasmid # 128085 ; http://n2t.net/addgene:128085 ; RRID:Addgene_128085) -
For your References section:
Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation. Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL Jr. Cell. 2016 Feb 25;164(5):922-36. doi: 10.1016/j.cell.2016.02.001. 10.1016/j.cell.2016.02.001 PubMed 26919429