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Addgene

CFP-CitA6-130-Venus
(Plasmid #128016)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128016 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Construction and testing of plasmid was done in BL21.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CitA
  • Species
    Klebsiella pneumoniae
  • Mutation
    see Depositor Comments

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene identified the following mutations: S323N, A375E, R556G
These are not expected to impact plasmid function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CFP-CitA6-130-Venus was a gift from Uwe Sauer (Addgene plasmid # 128016 ; http://n2t.net/addgene:128016 ; RRID:Addgene_128016)

  • For your References section:

    Engineering genetically encoded nanosensors for real-time in vivo measurements of citrate concentrations. Ewald JC, Reich S, Baumann S, Frommer WB, Zamboni N. PLoS One. 2011;6(12):e28245. doi: 10.1371/journal.pone.0028245. Epub 2011 Dec 2. 10.1371/journal.pone.0028245 PubMed 22164251
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