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Addgene

LN-entry+NLS
(Plasmid #128011)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128011 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified from pAcM
  • Total vector size (bp) 9923
  • Vector type
    LamdaN entry vector with NLS to generate tethering construct
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LN-3xFlag-SV40NLS
  • Species
    D. melanogaster (fly), Synthetic
  • Tag / Fusion Protein
    • LN-3xFlag-SV40NLS (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LN-entry+NLS was a gift from Julius Brennecke (Addgene plasmid # 128011 ; http://n2t.net/addgene:128011 ; RRID:Addgene_128011)

  • For your References section:

    The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J. Nat Struct Mol Biol. 2019 Aug;26(8):720-731. doi: 10.1038/s41594-019-0270-6. Epub 2019 Aug 5. 10.1038/s41594-019-0270-6 PubMed 31384064
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