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Addgene

ROPY-4A
(Plasmid #128005)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128005 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ROPY-4A
  • Species
    Synthetic
  • Mutation
    S16A, S25A, S38A, S63A in OP18

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ROPY-4A was a gift from Dorus Gadella (Addgene plasmid # 128005 ; http://n2t.net/addgene:128005 ; RRID:Addgene_128005)

  • For your References section:

    Combining optogenetics with sensitive FRET imaging to monitor local microtubule manipulations. Van Geel O, Cheung S, Gadella TWJ. Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.1038/s41598-020-62874-3. 10.1038/s41598-020-62874-3 PubMed 32265472
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