Pfis-cfp (ColE1)
(Plasmid
#127950)
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PurposeCFP fluorescent reporter for Fis
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127950 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneColE1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)MG1655
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePfis-cfp
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pfis-cfp (ColE1) was a gift from Mary Dunlop (Addgene plasmid # 127950 ; http://n2t.net/addgene:127950 ; RRID:Addgene_127950) -
For your References section:
Forecasting cell fate during antibiotic exposure using stochastic gene expression. Rossi NA, El Meouche I, Dunlop MJ. Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. eCollection 2019. 10.1038/s42003-019-0509-0 PubMed 31312728