p35S-GW-3Avi
(Plasmid
#127918)
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Purpose(Empty Backbone) Gateway destination vector for transcient expression of proteins with C-terminal 3_Avi in plant protoplast
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMD19-T
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Vector typePlant Expression
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Tag
/ Fusion Protein
- 3xAviTag (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- 3xAviTag (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gateway expression cassette from pEAQ-HT-DEST1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p35S-GW-3Avi was a gift from Silin Zhong (Addgene plasmid # 127918 ; http://n2t.net/addgene:127918 ; RRID:Addgene_127918) -
For your References section:
Reconstructing the maize leaf regulatory network using ChIP-seq data of 104 transcription factors. Tu X, Mejia-Guerra MK, Valdes Franco JA, Tzeng D, Chu PY, Shen W, Wei Y, Dai X, Li P, Buckler ES, Zhong S. Nat Commun. 2020 Oct 9;11(1):5089. doi: 10.1038/s41467-020-18832-8. 10.1038/s41467-020-18832-8 PubMed 33037196