pET30a-Tn5
(Plasmid
#127917)
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PurposeProkaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET30a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTn5
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MutationE54K, L372P
- Promoter T7
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Tag
/ Fusion Protein
- Mxe intein - Chitin-binding domain (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7ter (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Tn5 transposase from pTXB1-Tn5 (Addgene Plasmid #60240)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET30a-Tn5 was a gift from Silin Zhong (Addgene plasmid # 127917 ; http://n2t.net/addgene:127917 ; RRID:Addgene_127917) -
For your References section:
Reconstructing the maize leaf regulatory network using ChIP-seq data of 104 transcription factors. Tu X, Mejia-Guerra MK, Valdes Franco JA, Tzeng D, Chu PY, Shen W, Wei Y, Dai X, Li P, Buckler ES, Zhong S. Nat Commun. 2020 Oct 9;11(1):5089. doi: 10.1038/s41467-020-18832-8. 10.1038/s41467-020-18832-8 PubMed 33037196
