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Addgene

pET30a-HS-Tn5
(Plasmid #127916)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127916 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hyperstable Tn5
  • Alt name
    HS-Tn5
  • Mutation
    E54K, L372P
  • Promoter T7
  • Tags / Fusion Proteins
    • E coli elongation factor Ts (N terminal on insert)
    • Mxe intein - Chitin-binding domain (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Tn5 transposase from pTXB1-Tn5 (Addgene Plasmid #60240)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30a-HS-Tn5 was a gift from Silin Zhong (Addgene plasmid # 127916 ; http://n2t.net/addgene:127916 ; RRID:Addgene_127916)

  • For your References section:

    Reconstructing the maize leaf regulatory network using ChIP-seq data of 104 transcription factors. Tu X, Mejia-Guerra MK, Valdes Franco JA, Tzeng D, Chu PY, Shen W, Wei Y, Dai X, Li P, Buckler ES, Zhong S. Nat Commun. 2020 Oct 9;11(1):5089. doi: 10.1038/s41467-020-18832-8. 10.1038/s41467-020-18832-8 PubMed 33037196
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