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Addgene

pmNeonGreenHO-3xFLAG-G
(Plasmid #127914)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127914 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4035
  • Total vector size (bp) 4882
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    humanized mNeonGreen
  • Insert Size (bp)
    720
  • GenBank ID
  • Tag / Fusion Protein
    • mNeonGreen-3xFlag (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmNeonGreenHO-3xFLAG-G was a gift from Isei Tanida (Addgene plasmid # 127914 ; http://n2t.net/addgene:127914 ; RRID:Addgene_127914)
  • For your References section:

    Optimization of mNeonGreen for Homo sapiens increases its fluorescent intensity in mammalian cells. Tanida-Miyake E, Koike M, Uchiyama Y, Tanida I. PLoS One. 2018 Jan 17;13(1):e0191108. doi: 10.1371/journal.pone.0191108. eCollection 2018. 10.1371/journal.pone.0191108 PubMed 29342181