pmNeonGreenHO-3xFLAG-G
(Plasmid
#127914)
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Purposea mammalian expression backbone vector for humanized mNeonGreen-3xFlag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127914 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
- Backbone size w/o insert (bp) 4035
- Total vector size (bp) 4882
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehumanized mNeonGreen
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Insert Size (bp)720
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GenBank ID
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Tag
/ Fusion Protein
- mNeonGreen-3xFlag (C terminal on insert)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmNeonGreenHO-3xFLAG-G was a gift from Isei Tanida (Addgene plasmid # 127914 ; http://n2t.net/addgene:127914 ; RRID:Addgene_127914) -
For your References section:
Optimization of mNeonGreen for Homo sapiens increases its fluorescent intensity in mammalian cells. Tanida-Miyake E, Koike M, Uchiyama Y, Tanida I. PLoS One. 2018 Jan 17;13(1):e0191108. doi: 10.1371/journal.pone.0191108. eCollection 2018. 10.1371/journal.pone.0191108 PubMed 29342181
