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Human 3' HP1a AID GFP PuroR
(Plasmid #127906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSK
  • Vector type
    Donor plasmid
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HP1
  • Alt name
    CBX5
  • Alt name
    Heterochromatin Protein 1A
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Insert Size (bp)
    2097
  • Mutation
    Inserting GFP AID 2A Puro into mouse 3' Hp1a
  • GenBank ID
    NC_000012.12
  • Entrez Gene
    CBX5 (a.k.a. HEL25, HP1, HP1A, HP1alpha)
  • Tag / Fusion Protein
    • GFP, AID, PuroR

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human 3' HP1a AID GFP PuroR was a gift from Mark Groudine (Addgene plasmid # 127906 ; http://n2t.net/addgene:127906 ; RRID:Addgene_127906)

  • For your References section:

    HP1alpha is a chromatin crosslinker that controls nuclear and mitotic chromosome mechanics. Strom AR, Biggs RJ, Banigan EJ, Wang X, Chiu K, Herman C, Collado J, Yue F, Ritland Politz JC, Tait LJ, Scalzo D, Telling A, Groudine M, Brangwynne CP, Marko JF, Stephens AD. Elife. 2021 Jun 9;10. pii: 63972. doi: 10.7554/eLife.63972. 10.7554/eLife.63972 PubMed 34106828
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