pCHFLAGGR
(Plasmid
#1279)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS313
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGR
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Alt nameglucocorticoid receptor
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SpeciesR. norvegicus (rat)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nco I (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bysee comments
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCHFLAGGR was a gift from Susan Lindquist (Addgene plasmid # 1279 ; http://n2t.net/addgene:1279 ; RRID:Addgene_1279) -
For your References section:
Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways. Kimura Y, Yahara I, Lindquist S. Science 1995 Jun 2;268(5215):1362-5. 10.1126/science.7761857 PubMed 7761857