FH-TET3-HxDmut-pEF
(Plasmid #127896)

• Purpose: Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A
• Depositing Labs: Patrick Hogan, Anjana Rao
• Publication: Ko et al Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 2013 Apr 7.

Backbone

• Vector backbone: PEF1a
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6200
• Total vector size (bp): 11669
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TET3-HxDmut
• Species: H. sapiens (human)
• Insert Size (bp): 5469
• GenBank ID: NM_001287491.2
• Entrez Gene: TET3 (a.k.a. hCG_40738)
• Promoter: EF-1a
• Tag / Fusion Protein:
  • HA and Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH Reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry