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FH-TET3-CXXCmut-pEF
(Plasmid #127895)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127895 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PEF1a
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 11669
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TET3-CxxCmut
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5469
  • GenBank ID
    NM_001287491.2
  • Entrez Gene
    TET3 (a.k.a. hCG_40738)
  • Promoter EF-1a
  • Tag / Fusion Protein
    • HA and Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FH-TET3-CXXCmut-pEF was a gift from Patrick Hogan & Anjana Rao (Addgene plasmid # 127895 ; http://n2t.net/addgene:127895 ; RRID:Addgene_127895)

  • For your References section:

    Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain protein IDAX. Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A. Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 2013 Apr 7. 10.1038/nature12052 PubMed 23563267
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