MSCV-myc-CAR-2A-Thy1.1
(Plasmid #127890)

• Purpose: Expresses CART receptor along with tags.
• Depositing Lab: Anjana Rao
• Publication: Chen et al Nature. 2019 Mar;567(7749):530-534. doi: 10.1038/s41586-019-0985-x. Epub 2019 Feb 27.

Backbone

• Vector backbone: MSCV
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 5172
• Total vector size (bp): 7134
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: myc,
• Species: M. musculus (mouse)
• Insert Size (bp): 30

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: 5' MSCV

Gene/Insert 2

• Gene/Insert name: CAR
• Species: H. sapiens (human)
• Insert Size (bp): 1386

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: 5' MSCB

Gene/Insert 3

• Gene/Insert name: 2A
• Species: porcine
• Insert Size (bp): 57

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: 5' MSCV

Gene/Insert 4

• Gene/Insert name: Thy1.1
• Species: M. musculus (mouse)
• Insert Size (bp): 489

Cloning Information for Gene/Insert 4

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: 5' MSCV

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Pieced together from Cral June and Steve Rosenberg's papers
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry