pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE
(Plasmid #127868)

• Purpose: pAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoter
• Depositing Lab: Florian Freudenberg
• Publication: Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4):741-55. doi: 10.1016/j.euroneuro.2016.01.008. Epub 2016 Jan 28.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5349
• Total vector size (bp): 5751
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nos1
• Alt name: nNOS, NOS-I
• Species: M. musculus (mouse)
• Insert Size (bp): 402
• Mutation: Amino acids 1-133 only
• GenBank ID: NM_008712
• Entrez Gene: Nos1 (a.k.a. 2310005C01Rik, N-NOS, NC-NOS, NO, NOS, NOS-I, Nos-1, bNOS, nNOS)
• Promoter: hSyn
• Tags / Fusion Proteins:
  • 3xFLAG (C terminal on insert)
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: cactgccagcttcagcac
• 3′ sequencing primer: TTGGTCACCTTCAGCTTGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The backbone was obtained from pAAV-hSyn-hChR2(H134R)-mCherry, a gift from Karl Deisseroth (Addgene plasmid # 26976). mCherry was amplified by PCR from pAAV-hSyn-hChR2(H134R)-mCherry, a gift from Karl Deisseroth (Addgene plasmid # 26976).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry