pUC119 T7 hTRmin
(Plasmid
#127858)
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PurposeT7 RNA Pol for in vitro transcription
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127858 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC119
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC119 T7 hTRmin was a gift from Kathleen Collins (Addgene plasmid # 127858 ; http://n2t.net/addgene:127858 ; RRID:Addgene_127858) -
For your References section:
Human telomerase specialization for repeat synthesis by unique handling of primer-template duplex. Wu RA, Collins K. EMBO J. 2014 Apr 16;33(8):921-35. doi: 10.1002/embj.201387205. Epub 2014 Mar 11. 10.1002/embj.201387205 PubMed 24619002