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Addgene

pMX1418
(Plasmid #127856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    CLONTECH
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 6964
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IPNN-MCAK
  • Species
    C. griseus
  • Insert Size (bp)
    2243
  • Mutation
    mutation is I97N and P98N
  • GenBank ID
    U11790.2
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV promoter primer
  • 3′ sequencing primer SV40 polyA reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX1418 was a gift from Linda Wordeman (Addgene plasmid # 127856 ; http://n2t.net/addgene:127856 ; RRID:Addgene_127856)

  • For your References section:

    MCAK activity at microtubule tips regulates spindle microtubule length to promote robust kinetochore attachment. Domnitz SB, Wagenbach M, Decarreau J, Wordeman L. J Cell Biol. 2012 Apr 16;197(2):231-7. doi: 10.1083/jcb.201108147. Epub 2012 Apr 9. 10.1083/jcb.201108147 PubMed 22492725
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