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Addgene

HM3C NaVAe1_p pET24b
(Plasmid #127854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127854 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NaVAe1p
  • Species
    Alkalilimnicola ehrlichii
  • GenBank ID
    CP000453.1
  • Tags / Fusion Proteins
    • His
    • MBP
    • 3C Cleavage site

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HM3C NaVAe1_p pET24b was a gift from Dan Minor (Addgene plasmid # 127854 ; http://n2t.net/addgene:127854 ; RRID:Addgene_127854)

  • For your References section:

    Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation. Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL Jr. Cell. 2016 Feb 25;164(5):922-36. doi: 10.1016/j.cell.2016.02.001. 10.1016/j.cell.2016.02.001 PubMed 26919429
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