HM3C NaVSp1_p pET24b
(Plasmid
#127852)
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PurposeBacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127852 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET24b
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNaVSp1
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SpeciesSilicibacter pomeroyi
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GenBank IDAY392137.1
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Tags
/ Fusion Proteins
- His Tag
- MBP
- 3C Cleavage site
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HM3C NaVSp1_p pET24b was a gift from Dan Minor (Addgene plasmid # 127852 ; http://n2t.net/addgene:127852 ; RRID:Addgene_127852) -
For your References section:
Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation. Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL Jr. Cell. 2016 Feb 25;164(5):922-36. doi: 10.1016/j.cell.2016.02.001. 10.1016/j.cell.2016.02.001 PubMed 26919429