pBT271.361
(Plasmid
#127846)
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Purposeclone A3A-W98Y CBEs by SapI Golden Gate
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBT195
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameA3A-W98Y
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SpeciesH. sapiens (human)
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MutationW98Y
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site N/A (unknown if destroyed)
- 3′ cloning site N/A (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Along with pBT195, this plasmid can be used to clone pBT361 by SapI or LguI restriction/ligation (Golden Gate). pBT361 is mutagenic in bacteria and care must be taken during cloning, handling and propagation. Benchling map: https://benchling.com/s/seq-viWaBa6R2LjXykdEZnZo
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBT271.361 was a gift from David Liu (Addgene plasmid # 127846 ; http://n2t.net/addgene:127846 ; RRID:Addgene_127846) -
For your References section:
Continuous evolution of base editors with expanded target compatibility and improved activity. Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. 10.1038/s41587-019-0193-0 PubMed 31332326