pNH-TrxT-G-S10
(Plasmid
#127829)
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PurposeProduction of recombinant human ribosomal protein S10
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 127829 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNH-TrxT
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRPS10
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Alt nameRibosomal protein S10
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SpeciesH. sapiens (human)
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Entrez GeneRPS10 (a.k.a. DBA9, S10)
- Promoter T7
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Tag
/ Fusion Protein
- His6 - Thioredoxin - TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNH-TrxT-G-S10 was a gift from Ivanhoe Leung (Addgene plasmid # 127829 ; http://n2t.net/addgene:127829 ; RRID:Addgene_127829) -
For your References section:
An improved method for the heterologous production of soluble human ribosomal proteins in Escherichia coli. Correddu D, Montano Lopez JJ, Vadakkedath PG, Lai A, Pernes JI, Watson PR, Leung IKH. Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.1038/s41598-019-45323-8. 10.1038/s41598-019-45323-8 PubMed 31222068