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Addgene

pNH-TrxT-G-T136D-S15
(Plasmid #127824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNH-TrxT
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPS15
  • Alt name
    Ribosomal protein S15
  • Species
    H. sapiens (human)
  • Mutation
    T136D
  • Entrez Gene
    RPS15 (a.k.a. RIG, S15)
  • Promoter T7
  • Tag / Fusion Protein
    • His6 - Thioredoxin - TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNH-TrxT-G-T136D-S15 was a gift from Ivanhoe Leung (Addgene plasmid # 127824 ; http://n2t.net/addgene:127824 ; RRID:Addgene_127824)

  • For your References section:

    An improved method for the heterologous production of soluble human ribosomal proteins in Escherichia coli. Correddu D, Montano Lopez JJ, Vadakkedath PG, Lai A, Pernes JI, Watson PR, Leung IKH. Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.1038/s41598-019-45323-8. 10.1038/s41598-019-45323-8 PubMed 31222068
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