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Addgene

pCDNA6-LRRC52-V5-Flag-His
(Plasmid #127818)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127818 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA6
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LRRC52
  • Alt name
    BK channel auxiliary gamma subunit 2
  • Alt name
    Auxiliary subunit of the Slo3 channel
  • Species
    H. sapiens (human)
  • Entrez Gene
    LRRC52
  • Promoter CMV
  • Tag / Fusion Protein
    • V5, FLAG, 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA6-LRRC52-V5-Flag-His was a gift from Jiusheng Yan (Addgene plasmid # 127818 ; http://n2t.net/addgene:127818 ; RRID:Addgene_127818)

  • For your References section:

    Molecular basis for differential modulation of BK channel voltage-dependent gating by auxiliary gamma subunits. Li Q, Fan F, Kwak HR, Yan J. J Gen Physiol. 2015 Jun;145(6):543-54. doi: 10.1085/jgp.201511356. 10.1085/jgp.201511356 PubMed 26009545
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