GPI_2xmCherry
(Plasmid
#127812)
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PurposeHomo-dimer mCherry-mCherry expression vector with plasma membrane localization signal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemCherry-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameglycosylphosphatidylinositol-mCherry-mCherry
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GPI_2xmCherry was a gift from Salvatore Chiantia (Addgene plasmid # 127812 ; http://n2t.net/addgene:127812 ; RRID:Addgene_127812) -
For your References section:
Optimal fluorescent protein tags for quantifying protein oligomerization in living cells. Dunsing V, Luckner M, Zuhlke B, Petazzi RA, Herrmann A, Chiantia S. Sci Rep. 2018 Jul 13;8(1):10634. doi: 10.1038/s41598-018-28858-0. 10.1038/s41598-018-28858-0 PubMed 30006597