pNapin-eGFP
(Plasmid
#127797)
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Purpose(Empty Backbone) Napin Promoter driven eGFP expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCambia3300
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Modifications to backboneReplaced BASTA resistance with Napin-promoter driven eGFP selection in plants.
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Vector typePlant Expression
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Selectable markerseGFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNapin-eGFP was a gift from Scott Poethig (Addgene plasmid # 127797 ; http://n2t.net/addgene:127797 ; RRID:Addgene_127797) -
For your References section:
Traffic lines: new tools for genetic analysis in Arabidopsis thaliana. Wu G, Rossidivito G, Hu T, Berlyand Y, Poethig RS. Genetics. 2015 May;200(1):35-45. doi: 10.1534/genetics.114.173435. Epub 2015 Feb 23. 10.1534/genetics.114.173435 PubMed 25711279
