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pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A
(Plasmid #127790)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127790 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMH-HT
  • Backbone manufacturer
    Michael Hothorn
  • Backbone size w/o insert (bp) 5311
  • Total vector size (bp) 6262
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CtCHAD
  • Alt name
    Q8KE09
  • Species
    Chlorobium tepidum
  • Insert Size (bp)
    951
  • Mutation
    Residues 208-522 (CHAD domain), mutations in His253, Arg256, Arg260, Arg296, Arg418 and Tyr419 to Ala
  • Entrez Gene
    CT0884 (a.k.a. CT0884)
  • Tag / Fusion Protein
    • 6xHis tag + TEV cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TGCGTCCGGCGTAGAGGATCGAGATCT
  • 3′ sequencing primer CAGCTTCCTTTCGGGCTTTGTTAGCAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A was a gift from Michael Hothorn (Addgene plasmid # 127790 ; http://n2t.net/addgene:127790 ; RRID:Addgene_127790)

  • For your References section:

    Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules. Lorenzo-Orts L, Hohmann U, Zhu J, Hothorn M. Life Sci Alliance. 2019 May 27;2(3). pii: 2/3/e201900385. doi: 10.26508/lsa.201900385. Print 2019 Jun. 10.26508/lsa.201900385 PubMed 31133615
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