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pH7m34GW_RcCHAD-mCherry
(Plasmid #127784)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pH7m34GW
  • Backbone size w/o insert (bp) 8913
  • Total vector size (bp) 12564
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ubi10p:RcCHAD-mCherry
  • Alt name
    B9T8Q5
  • Species
    Ricinus communis
  • Insert Size (bp)
    3651
  • GenBank ID
    RCOM_0386350 EEF27758.1
  • Promoter Ubiquitin10 promoter + 5' UTR
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAGT
  • 3′ sequencing primer AACAGCTATGACCATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH7m34GW_RcCHAD-mCherry was a gift from Michael Hothorn (Addgene plasmid # 127784 ; http://n2t.net/addgene:127784 ; RRID:Addgene_127784)

  • For your References section:

    Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules. Lorenzo-Orts L, Hohmann U, Zhu J, Hothorn M. Life Sci Alliance. 2019 May 27;2(3). pii: 2/3/e201900385. doi: 10.26508/lsa.201900385. Print 2019 Jun. 10.26508/lsa.201900385 PubMed 31133615
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