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Addgene

pMH-HT_SsCHAD
(Plasmid #127783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127783 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMH-HT
  • Backbone manufacturer
    Michael Hothorn
  • Backbone size w/o insert (bp) 5313
  • Total vector size (bp) 5949
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SsCHAD
  • Alt name
    Q97YW1
  • Species
    Sulfolobus solfataricus
  • Insert Size (bp)
    636
  • Entrez Gene
    SULM_RS11045 (a.k.a. SULM_RS11045, SULM_11050)
  • Tag / Fusion Protein
    • 6xHis tag + TEV cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGCGTCCGGCGTAGAGGATCGAGATCT
  • 3′ sequencing primer CAGCTTCCTTTCGGGCTTTGTTAGCAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH-HT_SsCHAD was a gift from Michael Hothorn (Addgene plasmid # 127783 ; http://n2t.net/addgene:127783 ; RRID:Addgene_127783)

  • For your References section:

    Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules. Lorenzo-Orts L, Hohmann U, Zhu J, Hothorn M. Life Sci Alliance. 2019 May 27;2(3). pii: 2/3/e201900385. doi: 10.26508/lsa.201900385. Print 2019 Jun. 10.26508/lsa.201900385 PubMed 31133615
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