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Addgene

pU6-Sox3gRNA-T2
(Plasmid #127778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127778 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pU6
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sox3gRNAT2
  • Alt name
    sgRNA seq- TCTGCGCCGTGGACATCATG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmB1 (destroyed during cloning)
  • 3′ cloning site BsmB1 (destroyed during cloning)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-Sox3gRNA-T2 was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 127778 ; http://n2t.net/addgene:127778 ; RRID:Addgene_127778)

  • For your References section:

    Early chromatin shaping predetermines multipotent vagal neural crest into neural, neuronal and mesenchymal lineages. Ling ITC, Sauka-Spengler T. Nat Cell Biol. 2019 Dec;21(12):1504-1517. doi: 10.1038/s41556-019-0428-9. Epub 2019 Dec 2. 10.1038/s41556-019-0428-9 PubMed 31792380
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