pTK-EdnrbE1-Citrine
(Plasmid
#127773)
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PurposeEnhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127773 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTK
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Vector typeEnhancer reporter construct
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameednrbE1
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SpeciesG. gallus (chicken)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmB1 (destroyed during cloning)
- 3′ cloning site BsmB1 (destroyed during cloning)
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTK-EdnrbE1-Citrine was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 127773 ; http://n2t.net/addgene:127773 ; RRID:Addgene_127773) -
For your References section:
Early chromatin shaping predetermines multipotent vagal neural crest into neural, neuronal and mesenchymal lineages. Ling ITC, Sauka-Spengler T. Nat Cell Biol. 2019 Dec;21(12):1504-1517. doi: 10.1038/s41556-019-0428-9. Epub 2019 Dec 2. 10.1038/s41556-019-0428-9 PubMed 31792380