LentiUniversal-Puro
(Plasmid
#127749)
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Purpose(Empty Backbone) Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127749 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLentiUniversal-Puro
- Backbone size (bp) 10218
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Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter human U6
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsDH10B is also OK.
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For cloning guides, digest the plasmid with Esp3i for the backbone.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LentiUniversal-Puro was a gift from Giulio Draetta (Addgene plasmid # 127749 ; http://n2t.net/addgene:127749 ; RRID:Addgene_127749) -
For your References section:
Pooled library screening with multiplexed Cpf1 library. Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W, Deem A, Bristow C, Hart T, Draetta G. Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x. 10.1038/s41467-019-10963-x PubMed 31316073
