NLS-LucWT-GFP
(Plasmid
#127709)
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PurposeThe expressed ORF encodes nucleus location signal with GFP and WT Luciferase.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4600
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Vector typeMammalian Expression
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLuciferase
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Speciesfirefly
- Promoter CMV
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Tags
/ Fusion Proteins
- FLAG-NLS (N terminal on insert)
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6_primer
- 3′ sequencing primer pBABE_3_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NLS-LucWT-GFP was a gift from Xiaolu Yang (Addgene plasmid # 127709 ; http://n2t.net/addgene:127709 ; RRID:Addgene_127709) -
For your References section:
Assays for the Degradation of Misfolded Proteins in Cells. Guo L, Prall W, Yang X. J Vis Exp. 2016 Aug 28;(114). doi: 10.3791/54266. 10.3791/54266 PubMed 27684355
Map uploaded by the depositor.
