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Addgene

pSH86
(Plasmid #127706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Pmyo-3
  • Backbone size w/o insert (bp) 5582
  • Total vector size (bp) 6524
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP1-10
  • Species
    Synthetic
  • Insert Size (bp)
    942
  • Promoter myo-3

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TCCAAGCTTGCATGCgtgattatagtc
  • 3′ sequencing primer CTCTAGAGGCGCGCctcatttctagat
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSH86 was a gift from David M. Miller (Addgene plasmid # 127706 ; http://n2t.net/addgene:127706 ; RRID:Addgene_127706)

  • For your References section:

    NATF (Native And Tissue-Specific Fluorescence): A Strategy for Bright, Tissue-Specific GFP Labeling of Native Proteins in Caenorhabditis elegans. He S, Cuentas-Condori A, Miller DM. Genetics. 2019 Apr 5. pii: genetics.119.302063. doi: 10.1534/genetics.119.302063. 10.1534/genetics.119.302063 PubMed 30952669
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