pTRIPZ TREX1-KI
(Plasmid
#127701)
-
PurposeFor knocking in TREX1 in mouse - Doxycyclin inducible
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 127701 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepTRIPZ RFP-
- Backbone size w/o insert (bp) 13320
- Total vector size (bp) 13200
-
Modifications to backboneinsertion of mouse TREX1 cDNA between AgeI and MluI restriction sites
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namemouse TREX1 cDNA
-
gRNA/shRNA sequenceN/A
-
SpeciesM. musculus (mouse)
-
GenBank ID22040
-
Entrez GeneTrex1 (a.k.a. AU041952)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer atcgataccggtCATGGGCTCACAGACCCTG
- 3′ sequencing primer atcgatacgcgtTTACTGCCCAGGTGAGGC (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTRIPZ TREX1-KI was a gift from Sandra Demaria (Addgene plasmid # 127701 ; http://n2t.net/addgene:127701 ; RRID:Addgene_127701) -
For your References section:
DNA exonuclease Trex1 regulates radiotherapy-induced tumour immunogenicity. Vanpouille-Box C, Alard A, Aryankalayil MJ, Sarfraz Y, Diamond JM, Schneider RJ, Inghirami G, Coleman CN, Formenti SC, Demaria S. Nat Commun. 2017 Jun 9;8:15618. doi: 10.1038/ncomms15618. 10.1038/ncomms15618 PubMed 28598415