pcDNA3.1 5HT2A_NB2_1
(Plasmid #127690)

• Purpose: Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.
• Depositing Labs: Justin English, Bryan Roth
• Publication: English et al Cell. 2019 Jul 2. pii: S0092-8674(19)30622-1. doi: 10.1016/j.cell.2019.05.051.

Backbone

• Vector backbone: pCDNA3.1 hygro
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5HT2A_NB2
• Species: Synthetic

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.