S12.2_Bridge + Target
(Plasmid
#127671)
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Purpose(Empty Backbone) Ampicillin resistant multiplexing backbone with CRY2HA and mCherry
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127671 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneDerived from pUC19
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Backbone manufacturerCremins lab
- Backbone size (bp) 7761
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Vector typeMammalian Expression, CRISPR
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Selectable markersmCherry
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CRY2-HA-2A-mCherry and hU6 promoter with BbsI and BsaI sites
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
S12.2_Bridge + Target was a gift from Jennifer Phillips-Cremins (Addgene plasmid # 127671 ; http://n2t.net/addgene:127671 ; RRID:Addgene_127671) -
For your References section:
LADL: light-activated dynamic looping for endogenous gene expression control. Kim JH, Rege M, Valeri J, Dunagin MC, Metzger A, Titus KR, Gilgenast TG, Gong W, Beagan JA, Raj A, Phillips-Cremins JE. Nat Methods. 2019 Jun 24. pii: 10.1038/s41592-019-0436-5. doi: 10.1038/s41592-019-0436-5. 10.1038/s41592-019-0436-5 PubMed 31235883
Map uploaded by the depositor.
