LADL Anchor
(Plasmid #127664)

• Purpose: Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter
• Depositing Lab: Jennifer Phillips-Cremins
• Publication: Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s41592-019-0436-5. doi: 10.1038/s41592-019-0436-5.

Backbone

• Vector backbone: Derived from pUC19
• Backbone manufacturer: Cremins lab
• Backbone size w/o insert (bp): 3276
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: NEB10Beta is preferred for downstream applications.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9-CIBN-2A-Puro
• Species: Synthetic
• Insert Size (bp): 5517
• Mutation: Cas9 (D10A,H840A) mutant
• Promoter: EF1a
• Tag / Fusion Protein:
  • 3XFLAG (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: EF-1a Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • 2_ladl-anchor.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry