pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)
(Plasmid #127636)

• Purpose: Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)
• Depositing Lab: Friedrich Simmel
• Publication: Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657. doi: 10.1021/acs.nanolett.8b00526. Epub 2018 Mar 27.

Backbone

• Vector backbone: pSB1C3
• Backbone size w/o insert (bp): 2200
• Total vector size (bp): 32964963
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTurquoiuse2-Tat, YPet-PCP
• Species: Synthetic
• Insert Size (bp): 2500
• Tags / Fusion Proteins:
  • RNA binding peptide:Tat (C terminal on insert)
  • RNA binding peptide: PCP (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The inserts mTq2-Tat and YPet-PCP were designed by G.Grossi and M. Schwarz-Schilling and cloned by A. Dupin and M. Schwarz-Schilling. mTq2 sequence is from Goedhart, J. et al. Nat. Commun. 2012, 3, 751. Tat sequence is from Bayer, T. S. et al. RNA 2005, 11, 1848−57. YPet sequence is from Nguyen, A. W. and Daugherty, P. S. Nat. Biotechnol. 2005, 23, 355−360. PCP sequence is from Chao, J. A. et al. Nat. Struct. Mol. Biol. 2008, 15, 103−105. The peptide linker sequence between between mTq2 and Tat as well as for YPet and PCP is TRGGGGS.