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pSB1C3_mTq-Tat (c-plasmid)
(Plasmid #127635)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127635 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB1C3
  • Backbone size w/o insert (bp) 2200
  • Total vector size (bp) 3296
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTurquoise2
  • Alt name
    CFP
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Tags / Fusion Proteins
    • RNA binding peptide:Tat (C terminal on insert)
    • RNA binding peptide: PCP (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert mTq2-Tat was designed by G.Grossi and M. Schwarz-Schilling and cloned by A. Dupin and M. Schwarz-Schilling. mTq2 sequence is from Goedhart, J. et al. Nat. Commun. 2012, 3, 751. Tat sequence is from Bayer, T. S. et al. RNA 2005, 11, 1848−57. The peptide linker sequence between between YPet and PCP is TRGGGGS.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB1C3_mTq-Tat (c-plasmid) was a gift from Friedrich Simmel (Addgene plasmid # 127635 ; http://n2t.net/addgene:127635 ; RRID:Addgene_127635)

  • For your References section:

    Optimized Assembly of a Multifunctional RNA-Protein Nanostructure in a Cell-Free Gene Expression System. Schwarz-Schilling M, Dupin A, Chizzolini F, Krishnan S, Mansy SS, Simmel FC. Nano Lett. 2018 Apr 11;18(4):2650-2657. doi: 10.1021/acs.nanolett.8b00526. Epub 2018 Mar 27. 10.1021/acs.nanolett.8b00526 PubMed 29564885
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